Examines how reducing sitting time and increasing physical activity can be used to reverse aging.
We previously demonstrated that systemic administration of young blood can overcome age-related deficits in contextual fear conditioning and radial arm water maze learning in aged mice.
For proteome analysis purposes, eight tissues from PBS-injected and young sEV-treated aged mice were collected and subjected to isobaric tag for relative and absolute quantification (iTRAQ) quantitative proteomics analysis.
Physical Activity
Physical activity is a multidimensional endeavor that can be defined in various ways, including its type (the things people do), intensity (how much energy is expended), frequency and duration. Physical activity also depends upon its social context such as family/friend presence/absence; whether an activity takes place in an organized or unorganized fashion; as well as cultural expectations of what qualifies as “physical” activity.
Physical exercise has been proven to significantly enhance the health and functioning of older adults while decreasing frailty, yet current definitions of physical activity fail to encompass many important elements that contribute to its effectiveness. There exists room for a broader, more holistic definition of physical activity which could inform not only scientific study, but also policy statements, framing interventions in populations, teaching of physical activity in physical education programs as well as public health settings, among other possibilities.
Miranda Esmonde-White is an accomplished dancer, fitness instructor and author. Her books focus on wellness, health and aging. Miranda designed Essentrics as a dynamic stretching and strengthening workout featured on PBS Classical Stretch TV program.
Stress Management
Ultracentrifugation was used to isolate sEVs from male mice aged two months (2-month-old) and twenty months (20-month-old), and then they were diluted into PBS to achieve a concentration of 1.80 mg protein per microliter. Aged mice were administered seven doses of 200 ml PBS or two-month-old sEVs intravenously over two weeks and monitored for behavioral changes and overall body physiology. Aged mice treated with aged sEVs performed significantly better in both Morris water maze and grip strength tests compared with PBS-treated mice, while proteome-wide analysis of eight tissue samples from the spleen, lung, and hippocampus using uniform manifold approximation and projection (UMAP) annotation revealed clear differences in expression levels across groups.
To further elucidate how young sEVs could reverse aging, we conducted quantitative proteomics using iTRAQ technology on eight tissues from each group of mice. Our results revealed that on average 10,319 proteins were identified and quantified across both groups – with a heatmap showing differences in protein expression across groups.
